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Cell3™ Xtract: Cell-Free DNA (cfDNA) Extraction Kit

Cell3™ Xtract:

Cell-free DNA Extraction Kit

High-Sensitivity, Manual Cell-Free DNA Extraction

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Where Speed Meets Precision in Every Sample.
Effortless Workflow, No Special Equipment Needed.

Extract high-quality cell-free DNA (cfDNA) from plasma with Cell3™ Xtract, a manual spin-column kit designed for precision and efficiency. With no need for vacuum filtration, this streamlined workflow ensures optimal DNA recovery in just 35 μL elution volume, making it ideal for liquid biopsy, oncology research, NGS, and ddPCR applications.

The table of contents

Why Choose Cell3™ Xtract?

Flexible, Specialized Equipment-Free Workflow
  • Process 1-10 mL plasma using standard lab tools
NGS & ddPCR Ready
  • Low elution volume (35 μL) enhances sensitivity
  • Fragment sizes optimized for liquid biopsy and oncology research
Trusted Data Integrity
  • Validated with Agilent Tapestation and Qubit Fluorometry
Cell3™ Xtract Cell-Free (cfDNA) Extraction Kit

Key Features

Rapid Workflow

Process up to 10 mL of plasma in less than 90 minutes

No Carrier RNA Required

Ensure accurate quantification and library preparation

Spin Column Efficiency

Bypasses procedures which utilize magnets/vacuum devices, ideal for manual workflows

High Recovery

Maximize cfDNA for low-abundance variant detection

Performance Validation: Cell3™ Xtract vs. Competitors’ cfDNA Kits

To assess performance, Cell3™ Xtract was tested against four leading manual cfDNA extraction kits (Q, A, O, M), using plasma from a pregnant woman carrying a male fetus.

Spin Column Kits Comparison

  • Matched Company Q’s performance and outperformed Company A in fetal cfDNA extraction from 1 mL plasma (14-week gestation)
  • Unlike Company Q’s 50x higher DNA concentration (due to carrier RNA), Cell3™ Xtract delivered equivalent sensitivity without skewed results
Figure 1 CfDNA was extracted from 1 ml of plasma for each kit: the same sample from a male pregnancy at 14 weeks gestation was used. Quantitative PCR evaluation was conducted to detect and compare the extracted fetal cfDNA portion using the Cell3™ Direct: Fetal Sex Determination kit (Nonacus), which includes chromosome Y specific targets (TSPY, SRY, DAZ) and a control target (CCR5). Fragment size distribution was measured using high sensitivity reagents on the Tapestation 2200 (Agilent). DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

Bead-Based Kits Comparison

  • Outperformed Company M’s bead-based kit
  • Matched Company O in cfDNA recovery from 1 mL plasma (10-week gestation)
Figure 2 cfDNA was extracted from 1 ml of plasma for each kit: the same sample from a male pregnancy at 10 weeks gestation was used. Quantitative PCR, fragment size analysis and DNA concentration measurements were conducted as explained in Figure 1.
Ordering Information
ProductCatalog No.
Cell3 Xtract 16 SamplesNPRE_EXT_C3X_16
Cell3 Xtract 48 samplesPRE_EXT_C3X_48
Product Resources
Data Sheet

Cell3™ Xtract: Cell-free DNA Extraction Kit Data Sheet

Protocol

Cell3™ Xtract: cfDNA Extraction Protocol

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