Bead Xtract cfDNA kit

cfDNA Extraction Kit (for Manual and Automation Use)

Bead Xtract is designed to rapidly and efficiently extract cfDNA from up to 10 mL of plasma or serum using magnetic beads. Samples can be processed manually or using various automation platforms. Since no funnels or vacuum filtration are required, manual handling time can be minimized.

The table of contents

Simple and Scalable Extraction Method

The method is easy to use and adaptable to various applications and workflows. It allows for flexible adjustments in input volumes of serum or plasma, ranging from 0.5 to 10 mL, ensuring maximum recovery of cfDNA from samples (Table 1).

Table 1 The Bead Xtract cfDNA protocol enables a range of input volumes within the same kit.

Kit size	No. of extractions with 1 ml input	No. of extractions with 2 ml input	No. of extractions with 4 ml input
Small	20	10	5
Medium	200	100	50
Large	800	400	200

Minimized gDNA Contamination

The dissolution and binding buffers of Bead Xtract are optimized to maximize the recovery of small DNA fragments while minimizing contamination with gDNA. The extracted high-quality cfDNA is suitable for applications such as quantitative PCR (qPCR), droplet digital PCR (ddPCR), and next-generation sequencing (NGS) (Figure 1).

**Figure 1** Average DNA yield (ng) extracted from 1 ml plasma replicates using a range of different bead-based and spin-column based extraction kits. cfDNA was extracted following the manufacturer’s instructions and eluted into 50 µl of elution buffer. DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

High Performance

The strong binding capacity of the magnetic particles used in the Bead Xtract cfDNA kit allows for a higher DNA yield from plasma samples compared to cfDNA extraction kits from other major manufacturers (Figure 2). Additionally, it is possible to reduce the amount of particles needed to isolate the DNA, enabling a final elution volume of only 30-60 µL even when using 10 mL of plasma. This eliminates the need for vacuum filtration of the DNA, streamlining the protocol and ensuring that the final cfDNA sample concentration is suitable for applications such as NGS.

**Figure 2** Average DNA yield (ng) extracted from 1 ml plasma replicates using a range of different bead-based and spin-column based extraction kits. cfDNA was extracted following the manufacturer’s instructions and eluted into 50 µl of elution buffer. DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

Automation Protocol

Bead Xtract offers flexibility for execution both manually and through automation, accommodating a variety of sample processing volumes. The automated process from lysis to extraction has been validated on platforms such as the Hamilton ML STAR, and scripts are available for compatibility with various open-end liquid handlers. In fully automated cfDNA extraction, setup takes just 30 minutes, and processing 4 mL samples across 48 specimens with a 96-head Hamilton ML STAR can be completed in under 3 hours.

Ordering Information

Product	Catalog No.
Bead Xtract cfDNA, 20 (1 ml) extractions	PRE_EXT_BXC_20
Bead Xtract, cfDNA 200 (1 ml) extractions	PRE_EXT_BXC_200
Bead Xtract, cfDNA 800 (1 ml) extractions	PRE_EXT_BXC_800

Product Resources

Data Sheet

Bead Xtract cfDNA Extraction Kit Data Sheet

Protocol

Bead Xtract cfDNA Extraction Protocol