Bead Xtract
High-Efficiency Magnetic Bead cfDNA Extraction for Research and Clinical Use

Bead Xtract enables high-yield cfDNA extraction from plasma or serum using magnetic bead technology. Ideal for manual or automated workflows in liquid biopsy, qPCR, ddPCR, and NGS applications.

Unlock Superior cfDNA Recovery with Bead Xtract

The Bead Xtract revolutionizes cell-free DNA isolation with unmatched efficiency and scalability. Designed for both manual and automated workflows, this magnetic bead-based kit ensures rapid, high-purity cfDNA recovery from up to 10 mL of plasma or serum, ideal for cancer research, liquid biopsies, and non-invasive diagnostics. Since no funnels or vacuum filtration are required, manual handling time can be minimized.

The table of contents

Why Choose Bead Xtract?

Scalable Workflows

Process 0.5-10 mL samples with a single kit, ensuring flexibility for various research and clinical applications

Manual and Automation-Ready

Designed for both hands-on and high-throughput automated processing
Compatible with leading liquid handlers such as Hamilton ML STAR

High Purity, Minimal Contamination

Optimized buffers enhance small DNA fragment recovery while minimizing genomic DNA (gDNA) contamination for reliable downstream analysis

Superior Yield and Efficiency

Achieves higher cfDNA recovery compared to competitors’ bead-based and spin-column kits
Ensures optimal performance for qPCR, ddPCR, and NGS applications

Key Features

Flexible Input Volumes, Maximum Efficiency

Easily adapt to varying sample volumes without compromising yield, perfect for research and clinical settings

Kit size	No. of extractions with 1 ml input	No. of extractions with 2 ml input	No. of extractions with 4 ml input
Small	20	10	5
Medium	200	100	50
Large	800	400	200

Table 1 The Bead Xtract cfDNA protocol enables a range of input volumes within the same kit.

Minimized Contamination, Enhanced Purity

Optimized dissolution and binding buffers maximize small DNA fragment recovery
Reduces gDNA contamination, ensuring high-quality cfDNA suitable for qPCR, ddPCR, and NGS

**Figure 1** Average DNA yield (ng) extracted from 1 ml plasma replicates using a range of different bead-based and spin-column based extraction kits. cfDNA was extracted following the manufacturer’s instructions and eluted into 50 µl of elution buffer. DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

High Performance cfDNA Extraction

Magnetic bead technology enables superior DNA binding capacity, resulting in higher cfDNA yield from plasma samples
Optimized for efficient elution volumes (final elution volume of only 30–60 µL), even when processing 10 mL plasma samples
Eliminates vacuum filtration, streamlining the workflow for NGS-ready cfDNA

**Figure 2** Average DNA yield (ng) extracted from 1 ml plasma replicates using a range of different bead-based and spin-column based extraction kits. cfDNA was extracted following the manufacturer’s instructions and eluted into 50 µl of elution buffer. DNA concentration was determined using high sensitivity reagents on the Qubit 3.0 (Invitrogen).

Flexible Automation and Manual Workflow

Validated for automation on platforms such as the Hamilton ML STAR
Scripts available for various open-end liquid handlers
Automated cfDNA extraction
- Setup in just 30 minutes
- Processing 4 mL samples from 48 specimens in under 3 hours

Ordering Information

Product	Catalog No.
Bead Xtract cfDNA, 20 (1 ml) extractions	PRE_EXT_BXC_20
Bead Xtract, cfDNA 200 (1 ml) extractions	PRE_EXT_BXC_200
Bead Xtract, cfDNA 800 (1 ml) extractions	PRE_EXT_BXC_800

Product Resources

Data Sheet

Bead Xtract cfDNA Extraction Kit Data Sheet

Protocol

Bead Xtract cfDNA Extraction Protocol